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The second response cycle in NO synthesis involves O2 binding to ferrous NOS to form the Fe, IIO2 intermediate, followed by oxidation of NOHA to generate ferric enzyme, NO, citrulline, and water as products (Fig. 9) - UV/Vis/NIR. As drawn in Fig - https://allmyfaves.com/olisclarity1?tab=Olis%20Clarity. 9, the response might likewise involve electron transfer from H4B to the Fe, IIO2 intermediate
l9 As revealed in Fig. 9, this might be due to the fact that the H4B radical serve as an electron sink in a later point in the response, so as to allow NOS to, Fig. 9. A design for NORA oxidation by NOSoxy in the single turnover response. Oxygen binds to the ferrous protein to form the Fe, IIO2 species, which upon receiving an electron from H4B forms a putative hemeperoxo intermediate that blog here reacts with NOHA.